The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish.

ABSTRACT: Embryogenesis entails dramatic shifts in mRNA translation and turnover that reprogram gene expression during cellular proliferation and differentiation. Codon identity modulates mRNA stability during early vertebrate embryogenesis, but how the composition of tRNA pools is matched to translational demand is unknown. By quantitative profiling of tRNA repertoires in zebrafish embryos during the maternal-to-zygotic transition, we show that zygotic tRNA repertoires are established after the onset of gastrulation, succeeding the major wave of zygotic mRNA transcription. Maternal and zygotic tRNA pools are distinct, but their reprogramming does not result in a better match to the codon content of the zygotic transcriptome. Instead, we find that an increase in global translation at gastrulation se

SUBMITTER: Reimao-Pinto MM 

PROVIDER: S-EPMC11574265 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature