Exploring human pancreatic organoid modelling through single-cell RNA sequencing analysis.

ABSTRACT: Human organoids have been proposed to be powerful tools mimicking the physiopathological processes of the organs of origin. Recently, human pancreatic organoids (hPOs) have gained increasing attention due to potential theragnostic and regenerative medicine applications. However, the cellular components of hPOs have not been defined precisely. In this work, we finely characterized these structures, focusing first on morphology and identity-defining molecular features under long-term culture conditions. Next, we focused our attention on hPOs cell type composition using single-cell RNA sequencing founding a complex heterogeneity in ductal components, ranging from progenitor components to terminally differentiated ducts. Furthermore, an extensive comparison of human pancreatic organoids with p

SUBMITTER: Cherubini A 

PROVIDER: S-EPMC11574267 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature