Single-cell RNA sequencing analysis reveals the role of TXNDC5 in keloid formation.

ABSTRACT:

Objective

Thioredoxin domain-containing protein 5 (TXNDC5) is associated with fibrosis in a variety of organs, but its mechanism of action in keloid is unclear. In this study, we aimed to investigate the mechanism of TXNDC5 in keloid.

Material and methods

Single-cell RNA sequencing data of keloid and normal scar samples obtained from public databases were normalized and clustered using the Seurat package. Pathway enrich analysis was conducted using biological process enrichment analysis and Gene Set Enrichment Analysis (GSEA). In addition, TXNDC5 expression and its effects on migration and invasion of keloid fibroblasts (KFs) were validated based on cell function experiments.

Results

A total of five cell types were obtained. The KF clusters were further clustered into

SUBMITTER: Liu Z 

PROVIDER: S-EPMC11574684 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature