Repurposing a Fully Reducing Polyketide Synthase toward 2-Methyl Guerbet-like Lipids.

ABSTRACT: In nature, thousands of diverse and bioactive polyketides are assembled by a family of multifunctional, "assembly line" enzyme complexes called polyketide synthases (PKS). Since the late 20th century, there have been several attempts to decode, rearrange, and "reprogram" the PKS assembly line to generate valuable materials such as biofuels and platform chemicals. Here, the first module from Mycobacterium tuberculosis (Mt) PKS12, an unorthodox, "modularly iterative" PKS, was modified and repurposed toward the formation of 2-methyl Guerbet lipids, which have wide applications in industry. We established a robust method for the recombinant expression and purification of this modified module (named [M₁*]), and we demonstrated its ability to catalyze the formation of se

SUBMITTER: Herrera MA 

PROVIDER: S-EPMC11574752 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature