Mapping and tracing Grem1⁺ stromal cells in an <i>Apc</i> ^<i>Min/+</i> mouse utilizing cryopreserved intestinal sections prepared via modified Swiss-roll technique.

ABSTRACT: Grem1⁺ cancer-associated fibroblasts (CAFs) are crucial in colorectal cancer (CRC) development, yet technical challenges have limited understanding of their origins, spatiotemporal distribution, and potential roles. Here, we devised a custom mold, optimizing the gut Swiss-roll technique to create a single cryopreserved slide for comprehensive staining. Our integrated approach uncovered a marked increase in Grem1⁺ CAFs within Apc ^Min/+ mouse tumors at 12 weeks, compared to normal mucosa. Subsequent lineage tracing in Grem1-CreER ^T2 ; R26-LSL-tdTomato; Apc ^Min/+ mice revealed that most Grem1⁺ CAFs infiltrating the tumor core originated from Grem1⁺ intestinal reticular

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC11574797 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature