Bioenergetic shift and proteomic signature induced by lentiviral-transduction of GFP-based biosensors.

ABSTRACT: Fluorescent proteins (FPs) stand as pivotal tools extensively employed across diverse biological research endeavors in various model systems. However, long-standing concerns surround their use due to the numerous side effects associated with their expression. Recent investigations have brought to light the significance of hydrogen peroxide (H₂O₂) that is associated with the maturation process of green fluorescent protein (GFP) fluorophores. The structural and functional impairments associated with GFP expression are possibly linked to this amount of H₂O₂. In this study, we assess the impact of the GFP-based HyPer7 biosensor on cellular homeostasis and proteome changes, aiming to identify potential risks related to oxidative stress responses that

SUBMITTER: Barakat S 

PROVIDER: S-EPMC11574814 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature