Spatiotemporal characterization of extracellular matrix maturation in human artificial stromal-epithelial tissue substitutes.

ABSTRACT:

Background

Tissue engineering techniques offer new strategies to understand complex processes in a controlled and reproducible system. In this study, we generated bilayered human tissue substitutes consisting of a cellular connective tissue with a suprajacent epithelium (full-thickness stromal-epithelial substitutes or SESS) and human tissue substitutes with an epithelial layer generated on top of an acellular biomaterial (epithelial substitutes or ESS). Both types of artificial tissues were studied at sequential time periods to analyze the maturation process of the extracellular matrix.

Results

Regarding epithelial layer, ESS cells showed active proliferation, positive expression of cytokeratin 5, and low expression of differentiation markers, whereas SESS epithelium showed

SUBMITTER: Avila-Fernandez P 

PROVIDER: S-EPMC11575135 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature