Motif-guided identification of KRAS-interacting proteins.

ABSTRACT:

Background

For decades, KRAS has always been a huge challenge to the field of drug discovery for its significance in cancer progression as well as its difficulties in being targeted as an "undruggable" protein. KRAS regulates downstream signaling pathways through protein-protein interactions, whereas many interaction partners of KRAS remain unknown.

Results

We developed a workflow to computationally predict and experimentally validate the potential KRAS-interacting proteins based on the interaction mode of KRAS and its known binding partners. We extracted 17 KRAS-interacting motifs from all experimentally determined KRAS-containing protein complexes as queries to identify proteins containing fragments structurally similar to the queries in the human protein structure databas

SUBMITTER: Wu S 

PROVIDER: S-EPMC11575137 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature