Ascorbate peroxidase modulation confirms key role in Leishmania infantum oxidative defence.

ABSTRACT:

Background

Ascorbate peroxidase (APX) has emerged as a promising target for chemotherapy because of its absence in humans and crucial role in the antioxidant defence of trypanosomatids. APXs, which are class I haeme-containing enzymes, reduces hydrogen peroxide using ascorbate to produce water and monodehydroascorbate, thereby preventing cell damage caused by H₂O₂.

Methods

We aimed to create an APX-knockout L. infantum line using CRISPR/Cas9. Despite unsuccessful attempts at full knockouts, we achieved deletion of chromosomal copies post-APX episomal insertion, yielding LiΔchrAPX::LbAPX parasites. We performed phenotypic characterization to assess the impact of these genetic modifications, which included the determination of APX transcript expression le

SUBMITTER: Santos IFM 

PROVIDER: S-EPMC11575162 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature