Pan-genome-scale metabolic modeling of <i>Bacillus subtilis</i> reveals functionally distinct groups.

ABSTRACT: Bacillus subtilis is an important industrial and environmental microorganism known to occupy many niches and produce many compounds of interest. Although it is one of the best-studied organisms, much of this focus including the reconstruction of genome-scale metabolic models has been placed on a few key laboratory strains. Here, we substantially expand these prior models to pan-genome-scale, representing 481 genomes of B. subtilis with 2,315 orthologous gene clusters, 1,874 metabolites, and 2,239 reactions. Furthermore, we incorporate data from carbon utilization experiments for eight strains to refine and validate its metabolic predictions. This comprehensive pan-genome model enables the assessment of strain-to-strain differences related to nutrient utilization, fermentation

SUBMITTER: Neal M 

PROVIDER: S-EPMC11575223 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature