Transcriptomic and proteomic changes associated with cobalamin-dependent propionate production by the rumen bacterium <i>Xylanibacter ruminicola</i>.

ABSTRACT: Xylanibacter ruminicola is an abundant rumen bacterium that produces propionate in a cobalamin (vitamin B₁₂)-dependent manner via the succinate pathway. However, the extent to which this occurs across ruminal Xylanibacter and closely related bacteria, and the effect of cobalamin supplementation on the expression of propionate pathway genes and enzymes has yet to be investigated. To assess this, we screened 14 strains and found that almost all strains produced propionate when supplemented with cobalamin. X. ruminicola KHP1 was selected for further study, including complete genome sequencing, and comparative transcriptomics and proteomics of KHP1 cultures grown with and without supplemented cobalamin. The complete KHP1 genome was searched for cobalamin-binding

SUBMITTER: Mahoney-Kurpe SC 

PROVIDER: S-EPMC11575231 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature