Identification of critical residues for RNA binding of nairovirus nucleoprotein.

ABSTRACT: Orthonairovirus haemorrhagiae (CCHFV) is a tick-borne virus of the Orthonairovirus genus. CCHFV nucleoprotein binds to the viral genomic RNA, which is essential for transcription and replication. Based on structural analyses, several residues located in positively-charged regions of CCHFV nucleoprotein have been indicated to be important for RNA binding. We investigated the effects of each residue on RNA binding using Orthonairovirus hazaraense (HAZV), a surrogate model for CCHFV, to address the lack of detailed investigations. RNA immunoprecipitation assay revealed that the four basic amino acid residues (R59, R178, K414, and K465) are critical for RNA binding. All of these residues are located within the same positively-charged region. Basicity of these residues was also found to

SUBMITTER: Ohta K 

PROVIDER: S-EPMC11575266 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature