A temperature-induced metabolic shift in the emerging human pathogen Photorhabdus asymbiotica.

ABSTRACT: Photorhabdus is a bacterial genus containing both insect and emerging human pathogens. Most insect-restricted species display temperature restriction, unable to grow above 34°C, while Photorhabdus asymbiotica can grow at 37°C to infect mammalian hosts and cause Photorhabdosis. Metabolic adaptations have been proposed to facilitate the survival of this pathogen at higher temperatures, yet the biological mechanisms underlying these are poorly understood. We have reconstructed an extensively manually curated genome-scale metabolic model of P. asymbiotica (iEC1073, BioModels ID MODEL2309110001), validated through in silico gene knockout and nutrient utilization experiments with an excellent agreement between experimental data and model predictions. Integration of iE

SUBMITTER: Carter EL 

PROVIDER: S-EPMC11575385 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature