Genome editing of a rice CDP-DAG synthase confers multipathogen resistance.

ABSTRACT: The discovery and application of genome editing introduced a new era of plant breeding by giving researchers efficient tools for the precise engineering of crop genomes¹. Here we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice (Oryza sativa). We first isolated a lesion mimic mutant (LMM) from a mutagenized rice population. We then demonstrated that a 29-base-pair deletion in a gene we named RESISTANCE TO BLAST1 (RBL1) caused broad-spectrum disease resistance and showed that this mutation caused an approximately 20-fold reduction in yield. RBL1 encodes a cytidine diphosphate diacylglycerol synthase that is required for phospholipid biosynthesis². Mutation of RBL1 results in reduced levels of phosphatidylinositol and its

SUBMITTER: Sha G 

PROVIDER: S-EPMC11575942 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature