A complementary metaproteomic approach to interrogate microbiome cultivated from clinical colon biopsies.

ABSTRACT: The human gut microbiome plays a vital role in preserving individual health and is intricately involved in essential functions. Imbalances or dysbiosis within the microbiome can significantly impact human health and are associated with many diseases. Several metaproteomics platforms are currently available to study microbial proteins within complex microbial communities. In this study, we attempted to develop an integrated pipeline to provide deeper insights into both the taxonomic and functional aspects of the cultivated human gut microbiomes derived from clinical colon biopsies. We combined a rapid peptide search by MSFragger against the Unified Human Gastrointestinal Protein database and the taxonomic and functional analyses with Unipept Desktop and MetaLab-MAG. Across seven samples, we

SUBMITTER: Duong VA 

PROVIDER: S-EPMC11576236 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature