Refrigerated amniotic membrane maintains its therapeutic qualities for 48 hours.

ABSTRACT: During wound healing, the migration of keratinocytes is critical for wound closure. The application of amniotic membrane (AM) on wounds with challenging contexts (e.g., chronification and diabetic foot ulcer) has proven very successful. However, the use of AM for clinical practice has several restraints when applied to patients; the most important restriction is preserving AM's therapeutic properties between its thawing and application onto the patient's wound. Moreover, AM collection and processing requires a cleanroom, together with specialized staff and equipment, and facilities that are not usually available in many hospitals and healthcare units. In this publication, we kept previously cryopreserved AM at different temperatures (37°C, 20°C, and 4°C) in different media (DMEM high gluco

SUBMITTER: Stelling-Ferez J 

PROVIDER: S-EPMC11576280 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature