At what cost? The impact of bacteriophage resistance on the growth kinetics and protein synthesis of Escherichia coli.

ABSTRACT: Cost of bacteriophage resistance (COR) is important in explaining processes of diversification and coexistence in microbial communities. COR can be expressed in different traits, and the lack of universally applicable methods to measure fitness trade-offs makes COR challenging to study. Due to its fundamental role in growth, we explored protein synthesis as a target for quantifying COR. In this study, the growth kinetics of three genome-sequenced strains of phage-resistant Escherichia coli, along with the phage-susceptible wild-type, were characterized over a range of glucose concentrations. Bioorthogonal non-canonical amino acid tagging (BONCAT) was used to track differences in protein synthetic activity between the wild-type and phage-resistant E. coli. Two of the resistant strains, with

SUBMITTER: Landor LAI 

PROVIDER: S-EPMC11576411 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature