Molecular characterization of wheat dwarf virus isolates from Serbia based on complete genome sequences.

ABSTRACT:

Introduction

Wheat dwarf virus (WDV), the species Mastrevirus hordei of the genus Mastrevirus in the family Geminiviridae, is a cereal virus commonly detected in several European, African and Asian countries that causes economic losses.

Methods

In the spring of 2019, a severe outbreak of wheat dwarfing and yellowing was observed in many winter wheat crops across Serbia. A total of 161 samples were tested for the presence of WDV and other common wheat viruses using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). To obtain the complete genome of 23 selected isolates, several overlapping segments of the WDV genome were amplified and sequenced. A phylogenetic tree was constructed using the whole genome sequences of the WDV isolates id

SUBMITTER: Stankovic I 

PROVIDER: S-EPMC11576430 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature