Chromatin landscape dynamics during reprogramming towards human naive and primed pluripotency reveals the divergent function of PRDM1 isoforms.

ABSTRACT: Induced pluripotent stem cells (iPSCs) technology holds great potential in both scientific research and clinical applications. It enables the generation of naïve and primed iPSCs from various cell types through different strategies. Despite extensive characterizations of transcriptional and epigenetic factors, the intricacies of chromatin landscape dynamics during naïve and primed reprogramming, particularly in humans, remain poorly understood. In this study, we employed ATAC-seq and RNA-seq analyses to delineate and compare the chromatin landscape of naïve and primed pluripotency through the human secondary reprogramming system. Our investigations revealed several key transcriptional and epigenetic factors pivotal for reprogramming-associated chromatin remodeling. Notably, we found two is

SUBMITTER: Zhou J 

PROVIDER: S-EPMC11576854 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature