Control of spatio-temporal patterning via cell growth in a multicellular synthetic gene circuit.

ABSTRACT: A major goal in synthetic development is to build gene regulatory circuits that control patterning. In natural development, an interplay between mechanical and chemical communication shapes the dynamics of multicellular gene regulatory circuits. For synthetic circuits, how non-genetic properties of the growth environment impact circuit behavior remains poorly explored. Here, we first describe an occurrence of mechano-chemical coupling in synthetic Notch (synNotch) patterning circuits: high cell density decreases synNotch-gated gene expression in different cellular systems in vitro. We then construct, both in vitro and in silico, a synNotch-based signal propagation circuit whose outcome can be regulated by cell density. Spatial and temporal patterning outcomes of this circuit can be predict

SUBMITTER: Santorelli M 

PROVIDER: S-EPMC11577002 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature