Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts.

ABSTRACT: Embryonic stem cells possess the remarkable ability to self-organize into blastocyst-like structures upon induction. These stem cell-based embryo models serve as invaluable platforms for studying embryogenesis and therapeutic developments. Nevertheless, the specific intrinsic regulators that govern this potential for blastoid formation remain unknown. Here we demonstrate an intrinsic program that plays a crucial role in both blastoids and blastocysts across multiple species. We first establish metrics for grading the resemblance of blastoids to mouse blastocysts, and identify the differential activation of gene regulons involved in lineage specification among various blastoid grades. Notably, abrogation of nuclear receptor subfamily 1, group H, member 2 (Nr1h2) drastically reduces blastoid

SUBMITTER: Wong KW 

PROVIDER: S-EPMC11577042 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature