Rapid and Specific Detection of <i>Bacillus cereus</i> Using Phage Protein-Based Lateral Flow Assays.

ABSTRACT: Rapid and precise diagnostic techniques are essential for identifying foodborne pathogens, including Bacillus cereus (B. cereus), which poses significant challenges to food safety. Traditional detection methods are limited by long incubation times and high costs. In this context, gold nanoparticle (AuNP)-based lateral flow assays (LFAs) are emerging as valuable tools for rapid screening. However, the use of antibodies in LFAs faces challenges, including complex production processes, ethical concerns, or variability. Here, we address these challenges by proposing an innovative approach using bacteriophage-derived proteins for pathogen detection on LFAs. We used the engineered endolysin cell-wall-binding domain (CBD) and distal tail proteins (Dit) from bacteriophages that speci

SUBMITTER: Le Brun G 

PROVIDER: S-EPMC11577320 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature