Tracking Small Extracellular Vesicles Using a Minimally Invasive PicoGreen Labeling Strategy.

ABSTRACT: Extracellular vesicles (EVs) are cell-secreted lipid bilayer delimited particles that mediate cellular communication. These tiny sacs of cellular information play an important role in cell communication and alter the physiological process under both normal and pathological conditions. As such, tracking EVs can provide valuable information regarding the basic understanding of cell communication, the onset of early malignancy, and biomarker discovery. Most of the current EV-tracking strategies are invasive, altering the natural characteristics of EVs by modifying the lipid bilayer with lipophilic dyes or surface proteins with fluorescent reporters. The invasive labeling strategies could alter the natural processes of EVs and thereby have major limitations for functional studies. Here, we rep

SUBMITTER: Rayamajhi S 

PROVIDER: S-EPMC11577420 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature