Microbial single-cell applications under anoxic conditions.

ABSTRACT: The field of microbiology traditionally focuses on studying microorganisms at the population level. Nevertheless, the application of single-cell level methods, including microfluidics and imaging techniques, has revealed heterogeneity within populations, making these methods essential to understand cellular activities and interactions at a higher resolution. Moreover, single-cell sorting has opened new avenues for isolating cells of interest from microbial populations or complex microbial communities. These isolated cells can be further interrogated in downstream single-cell "omics" analyses, providing physiological and functional information. However, applying these methods to study anaerobic microorganisms under in situ conditions remains challenging due to their sensitivity to ox

SUBMITTER: Keating C 

PROVIDER: S-EPMC11577760 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature