Assessing horizontal gene transfer in the rhizosphere of <i>Brachypodium distachyon</i> using fabricated ecosystems (EcoFABs).

ABSTRACT: Horizontal gene transfer (HGT) is a major process by which genes are transferred between microbes in the rhizosphere. However, examining HGT remains challenging due to the complexity of mimicking conditions within the rhizosphere. Fabricated ecosystems (EcoFABs) have been used to investigate several complex processes in plant-associated environments. Here we show that EcoFABs are efficient tools to examine and measure HGT frequency in the rhizosphere. We provide the first demonstration of gene transfer via a triparental conjugation system in the Brachypodium distachyon rhizosphere in an EcoFAB using Pseudomonas putida KT2440 as both donor and recipient bacterial strain with the donor containing a mobilizable and non-self-transmissible plasmid. We observed that the frequency o

SUBMITTER: Priya S 

PROVIDER: S-EPMC11577780 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature