Characterization of A Bronchoscopically Induced Transgenic Lung Cancer Pig Model for Human Translatability.

ABSTRACT:

Background

There remains a need for animal models with human translatability in lung cancer (LC) research. Findings in pigs have high impact on humans due to similar anatomy and physiology. We present the characterization of a bronchoscopically-induced LC model in Oncopigs carrying inducible KRAS^G12D and TP53^R167H mutations.

Methods

Twelve Oncopigs underwent 29 injections via flexible bronchoscopy. Eighteen Adenovirus-Cre recombinase gene (AdCre) inductions were performed endobronchially (n=6) and transbronchially with a needle (n=12). Eleven control injections were performed without AdCre. Oncopigs underwent serial contrast-enhanced chest CT with clinical follow-up for 29 weeks. Following autopsy, lung and organ tissues underwent histopathology, immuno

SUBMITTER: Joshi K 

PROVIDER: S-EPMC11577786 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature