Development of a Limosilactobacillus reuteri therapeutic delivery platform with reduced colonization potential.

ABSTRACT: Bacterial biotherapeutic delivery vehicles have the potential to treat a variety of diseases. This approach obviates the need to purify the recombinant effector molecule, allows delivery of therapeutics in situ via oral or intranasal administration, and protects the effector molecule during gastrointestinal transit. Lactic acid bacteria have been broadly developed as therapeutic delivery vehicles though risks associated with the colonization of a genetically modified microorganism have so-far not been addressed. Here, we present an engineered Limosilactobacillus reuteri strain with reduced colonization potential. We applied a dual-recombineering scheme for efficient barcoding and generated mutants in genes encoding five previously characterized and four uncharacterized putati

SUBMITTER: Alexander LM 

PROVIDER: S-EPMC11577788 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature