METTL14 suppresses the expression of YAP1 and the stemness of triple-negative breast cancer.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) has pronounced stemness that is associated with relapse. N⁶-methyladenosine (m⁶A) plays a crucial role in shaping cellular behavior by modulating transcript expression. However, the role of m⁶A in TNBC stemness, as well as the mechanisms governing its abundance, has yet to be elucidated.

Methods

We analyzed proteomic and transcriptomic data derived from breast cancer cohorts, with an emphasis on m⁶A regulators. To unravel the role of m⁶A in TNBC, we employed RNA sequencing, methylated RNA immunoprecipitation sequencing, RNA immunoprecipitation, chromatin immunoprecipitation, and luciferase reporter assays with mesenchymal stem-like (MSL) TNBC models. The clinical relevance

SUBMITTER: Bai X 

PROVIDER: S-EPMC11577812 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature