Preservation of Murine Whole Eyes With Supplemented UW Cold Storage Solution: Anatomical Considerations.

ABSTRACT:

Purpose

Retinal ganglion cell (RGC) apoptosis and axon regeneration are the principal obstacles challenging the development of successful whole eye transplantation (WET). The purpose of this study was to create a neuroprotective cocktail that targets early events in the RGC intrinsic apoptotic program to stabilize RGCs in a potential donor eye.

Methods

University of Wisconsin (UW) solution was augmented with supplements known to protect RGCs. Supplements targeted tyrosine kinase signaling, histone deacetylase activity, K+ ion efflux, macroglial stasis, and provided energy support. Modified UW (mUW) solutions with individual supplements were injected into the vitreous of enucleated mouse eyes, which were then stored in cold UW solution for 24 hours. Histopathology, immunostai

SUBMITTER: Muench NA 

PROVIDER: S-EPMC11578148 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature