Spatial and temporal distribution of ribosomes in single cells reveals aging differences between old and new daughters of <i>Escherichia coli</i>.

ABSTRACT: Lineages of rod-shaped bacteria such as Escherichia coli exhibit a temporal decline in elongation rate in a manner comparable to cellular or biological aging. The effect results from the production of asymmetrical daughters, one with a lower elongation rate, by the division of a mother cell. The slower daughter compared to the faster daughter, denoted respectively as the old and new daughters, has more aggregates of damaged proteins and fewer expressed gene products. We have examined further the degree of asymmetry by measuring the density of ribosomes between old and new daughters and between their poles. We found that ribosomes were denser in the new daughter and also in the new pole of the daughters. These ribosome patterns match the ones we previously found for expressed gene pr

SUBMITTER: Chao L 

PROVIDER: S-EPMC11578582 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature