Distinct trafficking routes of polarized and non-polarized membrane cargoes in <i>Aspergillus nidulans</i>.

ABSTRACT: Membrane proteins are sorted to the plasma membrane via Golgi-dependent trafficking. However, our recent studies challenged the essentiality of Golgi in the biogenesis of specific transporters. Here, we investigate the trafficking mechanisms of membrane proteins by following the localization of the polarized R-SNARE SynA versus the non-polarized transporter UapA, synchronously co-expressed in wild-type or isogenic genetic backgrounds repressible for conventional cargo secretion. In wild-type, the two cargoes dynamically label distinct secretory compartments, highlighted by the finding that, unlike SynA, UapA does not colocalize with the late-Golgi. In line with early partitioning into distinct secretory carriers, the two cargoes collapse in distinct ER-Exit Sites (ERES) in a sec31

SUBMITTER: Sagia GM 

PROVIDER: S-EPMC11578586 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature