Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.

ABSTRACT:

Introduction

Very little is known about the role of macrophages as immune mediators during natural HIV infection. Humanized mice are an extremely valuable in vivo model for studying HIV pathogenesis. However, the presence of murine mononuclear phagocytes in these models represents a significant limitation for studying their human counterpart. Therefore, we have developed a novel humanized mouse model that allows selective depletion of human myeloid cells at a time point of our choosing.

Methods

We genetically engineered human hematopoietic stem and progenitor cells (HSPCs) to express an inducible caspase-9 (iCas9) suicide system under a synthetic myeloid promoter. Using these HSPCs, we generated humanized mice. iCasp9 induction in vivo resulted in selective hum

SUBMITTER: Baroncini L 

PROVIDER: S-EPMC11578737 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature