Simple and robust high-throughput serum proteomics workflow with low-microflow LC-MS/MS.

ABSTRACT: Clinical proteomics has substantially advanced in identifying and quantifying proteins from biofluids, such as blood, contributing to the discovery of biomarkers. The throughput and reproducibility of serum proteomics for large-scale clinical sample analyses require improvements. High-throughput analysis typically relies on automated equipment, which can be costly and has limited accessibility. In this study, we present a rapid, high-throughput workflow low-microflow LC-MS/MS method without automation. This workflow was optimized to minimize the preparation time and costs by omitting the depletion and desalting steps. The developed method was applied to data-independent acquisition (DIA) analysis of 235 samples, and it consistently yielded approximately 6000 peptides and 600 protein groups

SUBMITTER: Seo Y 

PROVIDER: S-EPMC11579186 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature