Optimized simple culture protocol for inducing mature myotubes from MYOD1-overexpressed human iPS cells.

ABSTRACT: The forced expression system of MYOD1, a master gene for myogenic differentiation, can efficiently and rapidly reproduce muscle differentiation of human induced pluripotent stem cells (hiPSCs). Despite these advantages of the MYOD1 overexpression system, developed myotubes are relatively immature and do not recapitulate several aspects of striated muscle fibers. Here, we developed a simple optimized protocol using an alternative culture medium for maximizing the advantages of the MYOD1 overexpression system, and successfully improved the formation of multinucleated mature myotubes within 10 days. In this study, we generated hiPSCs derived from healthy donors and an individual with congenial muscular dystrophy caused by LMNA mutation (laminopathy), and compared disease-associated phenotypes

SUBMITTER: Wada E 

PROVIDER: S-EPMC11579357 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature