Cryo-EM structures of apo-APC/C and APC/C^CDH1:EMI1 complexes provide insights into APC/C regulation.

ABSTRACT: APC/C is a multi-subunit complex that functions as a master regulator of cell division. It controls progression through the cell cycle by timely marking mitotic cyclins and other cell cycle regulatory proteins for degradation. The APC/C itself is regulated by the sequential action of its coactivator subunits CDC20 and CDH1, post-translational modifications, and its inhibitory binding partners EMI1 and the mitotic checkpoint complex. In this study, we took advantage of developments in cryo-electron microscopy to determine the structures of human APC/C^CDH1:EMI1 and apo-APC/C at 2.9 Å and 3.2 Å resolution, respectively, providing insights into the regulation of APC/C activity. The high-resolution maps allow the unambiguous assignment of an α-helix to the N-terminus of CDH1 (CDH1

SUBMITTER: Hofler A 

PROVIDER: S-EPMC11579458 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature