Characterizing CD38 expression in terminally differentiated B cells using variable lymphocyte receptor B tetramers.

ABSTRACT:

Introduction

CD38 is an ectoenzyme receptor found on hematopoietic cells and its expression is used in the flow cytometric analysis of sub-populations of circulating B cells among peripheral blood mononuclear cells (PBMC) to aid in diagnosing patients with different antibody production defects (AbD). Monoclonal antibodies derived from the sea lamprey Variable Lymphocyte Receptor B (VLRB) are emerging as an alternative to conventional mammalian antibodies. We hypothesized that VLRB MM3 (V-CD38) which specifically recognizes CD38 in a manner correlating with its enzymatic activity could identify terminally differentiated B cells in human PBMC. Here we investigate the ability of V-CD38 as a tool to diagnose patients with diverse immune abnormalities including AbD.

Methods

The e

SUBMITTER: Nair AG 

PROVIDER: S-EPMC11579616 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature