Robust Sequence Design Space for the Isothermal Exponential Amplification of Short Oligonucleotides.

ABSTRACT: Advances in isothermal amplification techniques have accelerated development in biosensing applications and the design of complex molecular devices. The exponential amplification reaction technique, or EXPAR, is uniquely positioned to process molecular information from short oligonucleotide strands (≈10 nucleotides length) typically encountered in molecular computing or microRNA detection. Despite its conceptual simplicity (requiring only a template strand and two enzymes), the issue of nonspecific background amplification has hindered broader adoption. In this work, a new system configuration is established at 37 °C to achieve significantly improved performance. Critical sequence motifs responsible for the excellent signal-to-background profile are identified and generalized as a universa

SUBMITTER: Ang YS 

PROVIDER: S-EPMC11579956 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature