Effect of zoledronic acid on muscle metabolism in mice with osteoporosis combined with sarcopenia.

ABSTRACT:

Objective

To investigate the effects of zoledronic acid on muscle metabolism in mice with osteoporosis and sarcopenia and elucidate the possible underlying mechanism.

Methods

Twenty-four 8-week-old male C57BL/6J mice were randomly divided into four groups: non-suspension (N-SUS), suspension (SUS), suspension + zoledronic acid (ZA), and suspension + PTH(PTH) groups. Equal doses of saline, zoledronic acid, and PTH were administered subcutaneously. After 4 weeks, the mice were sacrificed, and body weight and muscle mass (gastrocnemius and soleus) were measured, the right tibia of mice was taken for micro-CT examination, and the muscle specimens were analyzed using HE staining, ATPase staining, western blotting, and real-time PCR.

Results

Compared with the N-SUS group, th

SUBMITTER: Li W 

PROVIDER: S-EPMC11580508 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature