Characterization of Single-Cell Cis-regulatory Elements Informs Implications for Cell Differentiation.

ABSTRACT: Cis-regulatory elements govern the specific patterns and dynamics of gene expression in cells during development, which are the fundamental mechanisms behind cell differentiation. However, the genomic characteristics of single-cell cis-regulatory elements closely linked to cell differentiation during development remain unclear. To explore this, we systematically analyzed ∼250,000 putative single-cell cis-regulatory elements obtained from snATAC-seq analysis of the developing mouse cerebellum. We found that over 80% of these single-cell cis-regulatory elements show pleiotropic effects, being active in 2 or more cell types. The pleiotropic degrees of proximal and distal single-cell cis-regulatory elements are positively correlated with the density and diversity of transcription factor bindin

SUBMITTER: Ren YY 

PROVIDER: S-EPMC11580522 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature