Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha-4,11-Diene Production.

ABSTRACT: Yeast extract (YE) is a complex nutritional source associated with high performance on microbial production processes. However, its inherent compositional variability challenges its scalability. While prior efforts have focused on growth-associated products, the dynamics of growth-uncoupled production, which leads to higher production rates and conversion yields, still need to be explored. This production scenario is common in large-scale applications. This study presents a systematic approach to replace YE for the production of the terpene amorpha-4,11-diene in Escherichia coli. Sequential processing was successfully applied to identify glutamic acid, alanine, leucine, valine, isoleucine and glycine as the key amino acids (AAs) under slow-growth conditions. Thoroughly applying biomass ret

SUBMITTER: Castillo-Saldarriaga C 

PROVIDER: S-EPMC11580704 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature