Integrated Studies on Male Reproductive Toxicity of Decabromodiphenyl Ethane in Zebrafish Spermatozoa <i>Ex Vivo</i>, Male Zebrafish <i>in Vivo</i>, and GC-1 Cells <i>in Vitro</i>.

ABSTRACT:

Background

Legacy brominated flame retardants have been recognized as risky factors leading to declined sperm quality. The widespread utilization of decabromodiphenyl ethane (DBDPE) as a replacement for decabromodiphenyl ether has given rise to considerable concern over its potential risks to reproductive health.

Objectives

The objectives were to quickly determine whether DBDPE affects sperm quality upon ex vivo exposure, to reveal the reproductive outcomes and underlying molecular mechanisms using an in vivo zebrafish model exposed to DBDPE, and to validate the potential impact on DNA damage and energy metabolism balance in vitro.

Methods

Zebrafish spermatozoa were treated with DBDPE (0.01, 0.1, 1, 10μM) for 3 h, and the spermatozoa motility and

SUBMITTER: Yang L 

PROVIDER: S-EPMC11580837 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature