Influence of metal ions on the isothermal self-assembly of DNA nanostructures.

ABSTRACT: DNA nanostructures are typically assembled by thermal annealing in buffers containing magnesium. We demonstrate the assembly of DNA nanostructures at constant temperatures ranging from 4 °C to 50 °C in solutions containing different metal ions. The choice of metal ions and the assembly temperature influence the isothermal assembly of several DNA motifs and designed three-dimensional DNA crystals. Molecular dynamics simulations show more fluctuations of the DNA structure in select monovalent ions (Na⁺ and K⁺) compared to divalent ions (Mg²⁺ and Ca²⁺). A key highlight is the successful assembly of DNA motifs in nickel-containing buffer at temperatures below 40 °C, otherwise unachievable at higher temperatures, or using an annealing protocol. DNA na

SUBMITTER: Rodriguez A 

PROVIDER: S-EPMC11580887 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature