<i>FOXP</i> Genes Regulate Purkinje Cell Diversity in Cerebellar Development and Evolution.

ABSTRACT: Mammalian cerebellar development is thought to be influenced by distinct Purkinje cell (PC) subtypes. However, the degree of PC heterogeneity and the molecular drivers of this diversity have remained unclear, hindering efforts to manipulate PC diversification and assess its role in cerebellar development. Here, we demonstrate the critical role of Foxp genes in cerebellar development by regulating PC diversification. We identified 11 PC subtypes in the embryonic mouse cerebellum through single-cell RNA sequencing. Using a novel unsupervised method, we mapped these subtypes in three-dimensional space, revealing discrete PC subtypes predictive of adult cerebellar organization, including longitudinal stripes and lobules. These subtypes exhibit unique combinations of Foxp1, Fox

SUBMITTER: Khouri-Farah N 

PROVIDER: S-EPMC11581015 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature