Miro1 expression alters global gene expression, ERK1/2 phosphorylation, oxidation, and cell cycle progression.

ABSTRACT: Subcellular mitochondrial positioning in cells is necessary for localized energy and signaling requirements. Mitochondria are strategically trafficked throughout the cytoplasm via the actin cytoskeleton, microtubule motor proteins, and adaptor proteins. Miro1, an outer mitochondrial membrane adaptor protein, is necessary for attachment of mitochondria to microtubule motor proteins for trafficking. Previous work showed when Miro1 is deleted (Miro1^-/-) from mouse embryonic fibroblasts (MEFs), the mitochondria become sequestered to the perinuclear space, disrupting subcellular energy and reactive oxygen species gradients. Here, we show that Miro1^-/- MEFs grow slower compared to Miro1^+/+ and Miro1^-/- MEFs stably re-expressing the Myc-Miro1 plasmid. M

SUBMITTER: Shannon N 

PROVIDER: S-EPMC11581026 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature