Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries.

ABSTRACT:

Context

Estrogen receptor α (ERα) is a key regulator of reproductive function, particularly in ovarian development and function, yet the specifics of its role at the molecular level remain unclear.

Aims

The study aims to elucidate the molecular mechanisms of ERα-regulated transcriptional dynamics in ovarian cells using ERα knockout (αERKO) mice created via CRISPR/Cas9.

Methods

Single-cell RNA sequencing (scRNA-seq) was used to compare transcriptomes from individual ovarian cells in both wild type and αERKO mice. Bioinformatics analyses identified distinct cell populations and their transcriptional profiles post ERα deletion.

Key results

Distinct oocyte and granulosa cell populations were identified, with ERα deletion disrupting the regulation of genes linked to

SUBMITTER: Hu Q 

PROVIDER: S-EPMC11581351 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature