Confocal Microscopy Analysis of Protein Sorting to Plasmodesmata in Nicotiana benthamiana.

ABSTRACT: Plasmodesmata are membranous nanopores that connect the cytoplasm of adjacent plant cells and enable the cell-to-cell trafficking of nutrients, macromolecules, as well as invading viruses. Plasmodesmata play fundamental roles in the regulation of intercellular communication, contributing to plant development, environmental responses, and interactions with viral pathogens. Discovering plasmodesmal localization of plant or viral proteins could provide useful functional information about the protein and is important for understanding the mechanisms of plant-virus interactions. To facilitate these studies, we describe a protocol for confocal microscopy-based analysis of different plasmodesmal targeting proteins to select the best plasmodesmal marker for studying the virus-plasmodesmata interac

SUBMITTER: Kan Y 

PROVIDER: S-EPMC11581684 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature