Calcium nanoparticles target and activate T cells to enhance anti-tumor function.

ABSTRACT: Calcium signaling plays a crucial role in the activation of T lymphocytes. However, modulating calcium levels to control T cell activation in vivo remains a challenge. In this study, we investigate T cell activation using 12-myristate 13-acetate (PMA)-encapsulated CaCO₃ nanoparticles. We find that anti-PD-1 antibody-conjugated CaCO₃ nanoparticles can be internalized by T cells via receptor-mediated endocytosis and then gradually release calcium. This results in an increase in cytosolic calcium, which triggers the activation of NFAT and NF-κB pathways, especially when the surface of the CaCO₃ nanoparticles is loaded with PMA. Animal studies demonstrate that the PMA-loaded calcium nanoparticles enhance the activation and proliferation of cytotoxic T cells, le

SUBMITTER: Yang W 

PROVIDER: S-EPMC11582315 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature