3D Humanized Bioprinted Tubulointerstitium Model to Emulate Renal Fibrosis In Vitro.

ABSTRACT: Chronic kidney disease (CKD) leads to a gradual loss of kidney function, with fibrosis as pathological endpoint, which is characterized by extracellular matrix (ECM) deposition and remodeling. Traditionally, in vivo models are used to study interstitial fibrosis, through histological characterization of biopsy tissue. However, ethical considerations and the 3Rs (replacement, reduction, and refinement) regulations emphasizes the need for humanized 3D in vitro models. This study introduces a bioprinted in vitro model which combines primary human cells and decellularized and partially digested extracellular matrix (ddECM). A protocol was established to decellularize kidney pig tissue and the ddECM was used to encapsulate human renal cells. To investigate fibrosis progression, cells were treat

SUBMITTER: Addario G 

PROVIDER: S-EPMC11582511 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature