Environmentally Controlled Microfluidic System Enabling Immune Cell Flow and Activation in an Endothelialised Skin-On-Chip.

ABSTRACT: Integration of reconstructed human skin (RhS) into organ-on-chip (OoC) platforms addresses current limitations imposed by static culturing. This innovation, however, is not without challenges. Microfluidic devices, while powerful, often encounter usability, robustness, and gas bubble issues that hinder large-scale high-throughput setups. This study aims to develop a novel re-usable multi-well microfluidic adaptor (MMA) with the objective to provide a flexible tool for biologists implementing complex 3D biological models (e.g., skin) while enabling simultaneous user control over temperature, medium flow, oxigen (O₂), nitrogen (N₂), and carbon dioxide (CO₂) without the need for an incubator. The presented MMA device is designed to be compatible with standard,

SUBMITTER: Michielon E 

PROVIDER: S-EPMC11582514 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature