Genome-wide profiling of DNA repair proteins in single cells.

ABSTRACT: Accurate repair of DNA damage is critical for maintenance of genomic integrity and cellular viability. Because damage occurs non-uniformly across the genome, single-cell resolution is required for proper interrogation, but sensitive detection has remained challenging. Here, we present a comprehensive analysis of repair protein localization in single human cells using DamID and ChIC sequencing techniques. This study reports genome-wide binding profiles in response to DNA double-strand breaks induced by AsiSI, and explores variability in genomic damage locations and associated repair features in the context of spatial genome organization. By unbiasedly detecting repair factor localization, we find that repair proteins often occupy entire topologically associating domains, mimicking variabili

SUBMITTER: de Luca KL 

PROVIDER: S-EPMC11582664 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature